Conversion of Dihydroflavonols to Flavonols with Enzyme Extracts from Flower Buds of Matthiola incana R. Br

نویسندگان

  • R. Spribille
  • G. Forkmann
چکیده

Dioxygenase, Flavonoid Biosynthesis, Flavonols, Matthiola incana Soluble enzyme preparations from flower buds of Matthiola incana catalysed the conversion of dihydrokaempferol to kaempferol and of dihydroquercetin to quercetin. The reaction required 2-oxoglutarate, ascorbate and Fe2+ as cofactors and had a pH-optimum at about 7.0. Highest enzyme activity was already present in the youngest buds followed by a rapid decline during bud and flower development. Furthermore, a substantial correlation was observed between the enzyme activity for flavonol formation and the flavonol content of the buds and flowers.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana R. Br

Anthocyanins, Flavonoids, Chalcone Synthase, Genetic Control, Matthiola incana Chalcone synthase activity was demonstrated in enzyme preparations from flowers of defined genotypes of Matthiola incana (stock). The product formed from 4-coumaroyl-CoA and malonylCoA was naringenin and not the isomeric chalcone, because chalcone isomerase was also present in the reaction mixture. Chalcone synthase ...

متن کامل

Genetic and Biochemical Studies on the Conversion of Dihydroflavonols to Flavonols in Flowers of Petunia hybrida

Dioxygenase, Flavonoid Biosynthesis, Flavonols, Genetic Control, Petunia hybrida Soluble enzyme preparations from flower buds of Petunia hybrida catalyzed the conversion of dihydroflavonols to flavonols. Dihydrokaempferol and dihydroquercetin were readily converted to the respective flavonols, whereas dihydromyricetin was a poor substrate. The reaction required 2-oxoglutarate, ascorbate and Fe2...

متن کامل

Flavonol Synthase Activity and the Regulation of Flavonol and Anthocyanin Biosynthesis during Flower Development in Dianthus caryophyllus L. (Carnation)

Flavonol synthase (FLS) was demonstrated in crude extracts from flower buds o f Dianthus caryophyllus (carnation). The enzyme catalyzed the conversion o f dihydrokaempferol and dihydroquercetin to kaempferol and quercetin, respectively. The reaction required 2-oxoglutarate, ferrous ion and ascorbate as co-factors and had a pH optimum at about 7.4. The demonstration o f FLS activity allowed comp...

متن کامل

Micropropagation of Matthiola incana using BA and IBA. Behzad Kaviani

Matthiola incana (Brassicaceae) is an important ornamental plant, which is used as cut flower, pot plant, and landscape. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Micropropagation by organogenesis is an efficient in vitro propagation method for Matthiola incana. Starting from seeds, apical bud ...

متن کامل

Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3- and Flavonoid 3'-Hydroxylases

Anthocyanins, Flavonoids, Biosynthesis, Flavanon 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Matthiola incana Enzyme preparations from flowers of defined genotypes o f Matthiola incana contain two dif­ ferent hydroxylases for hydroxylation of naringenin in the 3and 3'-position, respectively. The 3-hydroxylase is a soluble enzyme and requires as cofactors 2-oxoglutarate, Fe2+ and ascorbate. Besides...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2013